فهرست مطالب
Title
Copyright
Contents
Chapter 1 Introduction
References
Chapter 2 Fundamentals of Biological Nanopore Electrochemistry
2.1 Basic Knowledge of Biological Electrochemistry in Nanopore Confined Spaces
2.2 Design a Single Biomolecule Sensing Interface
2.3 Aerolysin-based Single Biomolecule Interface
2.3.1 The Structure of an Aerolysin Nanopore
2.3.2 Expression and Purification of Aerolysin
2.3.3 Manipulation of Sensing Interface of Aerolysin
2.4 α-Hemolysin-based Single Biomolecule Interface
2.4.1 The Structure of an α-Hemolysin Nanopore
2.4.2 Expression and Purification of α-Hemolysin
2.5 Membrane Formation to Support Pore-forming Molecules
References
Chapter 3 Single-biomolecule Interface for Advanced Single Molecular Sensors
3.1 Nucleotide Detection
3.1.1 Nucleotide Detection at the Single-biomolecule Interface
3.1.2 Modulation the Translocation via Single-molecule Conformational Changes
3.1.3 Localize the DNA Targets at the Hot-spot for High Sensitivity
3.1.4 Direct Detection of Hetero-nucleotide via Ionic Current Discrimination
3.1.5 Uncovering the Sensing Region of an Aerolysin Nanopore
3.2 Peptide Sensing with Biological Nanopores
3.2.1 Case Study I: Peptide/Protein Conformation and Folding/Unfolding Detection
3.2.2 Case Study II: Peptide/Protein Translocation Analysis
3.2.3 Case Study III: Pathogenic Related Peptide/Protein Investigation
3.2.4 Case Study IV: Enzymatic Kinetics Monitoring
3.3 Photo-responsive Molecule Analysis
3.3.1 Case Study I: Monitoring the Photo-responsive Molecule Induced Molecular Processes
3.3.2 Case Study II: Directly Determination of Configuration of a Single Photo-responsive Oligonucleotide
3.3.3 Case Study III: Regulating the Translocation Speed of Single-molecule Train Using Photo-responsive Molecule
3.4 Host-Guest Interaction Analysis
3.4.1 Case Study I: Detection of Self-assembly Process of Host and Guest Molecules
3.4.2 Case Study II: Measurement of Assembly Behaviors of Supramolecular Polymers
3.5 Measurement of Covalent Bonding at the Single-molecule Level
3.6 Detection of Small Biomolecule
3.6.1 Case Study I: Transient Conformation Measurement of Flavin Adenine Dinucleotide (FAD)
3.6.2 Case Study II: Antibiotic Molecule Analysis by Biological Nanopores
Acknowledgements
References
Chapter 4 Solid-state Nanopore Confinement for Single Molecule Detection
4.1 Fabrication Methods
4.2 Real-time Monitoring of the DNA Unzipping Process
4.3 DNA-based Detection of Target Proteins
4.4 The Dynamics of Individual Protein–DNA Complexes Docked in a Confined Space
4.5 Confining Optical Enhancement Inside Nanopores
4.5.1 Fabrication of Optical Nanopores
4.5.2 An Integrated System for Optical and Electrical Detection for a Scattering Nanopore
4.5.3 Optical Nanopore-based Sensing Application
References
Chapter 5 A Confined Nanopipette: From Fundamental to Application
5.1 General Preparation and Characterization
5.2 Further Modification for Advanced Sensing
5.3 The Nanopipette-based Wireless Nanopore Electrode (WNE)
5.4 Electrochemical Application of the Confined Nanopipette
5.4.1 Single Nanoparticle Sensing in the Nanopore Confined Space
5.4.2 Small Biomolecules Detection with a Nanopipette
5.5 Application of a Nanopipette by Combining with Optical Methods
5.5.1 Visualization of Single Molecule Translocation Through the Nanopipette
5.5.2 Electrochemically Controlling Small-volume Delivery with a Nanopipette
5.5.3 Real-time Detection in a Single Living Cell with a Nanopipette
5.6 Conclusion and Outlook
Acknowledgements
References
Chapter 6 Instrumentation and Data Algorithm for Single Molecule Sensing
6.1 Challenges in Nanopore Instrumentation and Data Processing
6.2 Design Principles for Nanopore Electrochemical Instrumentation
6.3 Setups for Nanopore Electrochemical Instrumentation
6.3.1 Setups Based on Patch-clamp System
6.3.2 Setups Based on “Cube” Electrochemical Instrumentation
6.3.3 Other Commercialized Setups
6.4 Integrated Chips for Nanopore Electrochemical Instrumentation
6.5 Data Analysis in Nanopore Electrochemistry
6.6 Intelligent Big Data for Nanopore Electrochemistry
Acknowledgements
References
Chapter 7 Summary and Outlook
Perspective
References
Subject Index
Copyright
Contents
Chapter 1 Introduction
References
Chapter 2 Fundamentals of Biological Nanopore Electrochemistry
2.1 Basic Knowledge of Biological Electrochemistry in Nanopore Confined Spaces
2.2 Design a Single Biomolecule Sensing Interface
2.3 Aerolysin-based Single Biomolecule Interface
2.3.1 The Structure of an Aerolysin Nanopore
2.3.2 Expression and Purification of Aerolysin
2.3.3 Manipulation of Sensing Interface of Aerolysin
2.4 α-Hemolysin-based Single Biomolecule Interface
2.4.1 The Structure of an α-Hemolysin Nanopore
2.4.2 Expression and Purification of α-Hemolysin
2.5 Membrane Formation to Support Pore-forming Molecules
References
Chapter 3 Single-biomolecule Interface for Advanced Single Molecular Sensors
3.1 Nucleotide Detection
3.1.1 Nucleotide Detection at the Single-biomolecule Interface
3.1.2 Modulation the Translocation via Single-molecule Conformational Changes
3.1.3 Localize the DNA Targets at the Hot-spot for High Sensitivity
3.1.4 Direct Detection of Hetero-nucleotide via Ionic Current Discrimination
3.1.5 Uncovering the Sensing Region of an Aerolysin Nanopore
3.2 Peptide Sensing with Biological Nanopores
3.2.1 Case Study I: Peptide/Protein Conformation and Folding/Unfolding Detection
3.2.2 Case Study II: Peptide/Protein Translocation Analysis
3.2.3 Case Study III: Pathogenic Related Peptide/Protein Investigation
3.2.4 Case Study IV: Enzymatic Kinetics Monitoring
3.3 Photo-responsive Molecule Analysis
3.3.1 Case Study I: Monitoring the Photo-responsive Molecule Induced Molecular Processes
3.3.2 Case Study II: Directly Determination of Configuration of a Single Photo-responsive Oligonucleotide
3.3.3 Case Study III: Regulating the Translocation Speed of Single-molecule Train Using Photo-responsive Molecule
3.4 Host-Guest Interaction Analysis
3.4.1 Case Study I: Detection of Self-assembly Process of Host and Guest Molecules
3.4.2 Case Study II: Measurement of Assembly Behaviors of Supramolecular Polymers
3.5 Measurement of Covalent Bonding at the Single-molecule Level
3.6 Detection of Small Biomolecule
3.6.1 Case Study I: Transient Conformation Measurement of Flavin Adenine Dinucleotide (FAD)
3.6.2 Case Study II: Antibiotic Molecule Analysis by Biological Nanopores
Acknowledgements
References
Chapter 4 Solid-state Nanopore Confinement for Single Molecule Detection
4.1 Fabrication Methods
4.2 Real-time Monitoring of the DNA Unzipping Process
4.3 DNA-based Detection of Target Proteins
4.4 The Dynamics of Individual Protein–DNA Complexes Docked in a Confined Space
4.5 Confining Optical Enhancement Inside Nanopores
4.5.1 Fabrication of Optical Nanopores
4.5.2 An Integrated System for Optical and Electrical Detection for a Scattering Nanopore
4.5.3 Optical Nanopore-based Sensing Application
References
Chapter 5 A Confined Nanopipette: From Fundamental to Application
5.1 General Preparation and Characterization
5.2 Further Modification for Advanced Sensing
5.3 The Nanopipette-based Wireless Nanopore Electrode (WNE)
5.4 Electrochemical Application of the Confined Nanopipette
5.4.1 Single Nanoparticle Sensing in the Nanopore Confined Space
5.4.2 Small Biomolecules Detection with a Nanopipette
5.5 Application of a Nanopipette by Combining with Optical Methods
5.5.1 Visualization of Single Molecule Translocation Through the Nanopipette
5.5.2 Electrochemically Controlling Small-volume Delivery with a Nanopipette
5.5.3 Real-time Detection in a Single Living Cell with a Nanopipette
5.6 Conclusion and Outlook
Acknowledgements
References
Chapter 6 Instrumentation and Data Algorithm for Single Molecule Sensing
6.1 Challenges in Nanopore Instrumentation and Data Processing
6.2 Design Principles for Nanopore Electrochemical Instrumentation
6.3 Setups for Nanopore Electrochemical Instrumentation
6.3.1 Setups Based on Patch-clamp System
6.3.2 Setups Based on “Cube” Electrochemical Instrumentation
6.3.3 Other Commercialized Setups
6.4 Integrated Chips for Nanopore Electrochemical Instrumentation
6.5 Data Analysis in Nanopore Electrochemistry
6.6 Intelligent Big Data for Nanopore Electrochemistry
Acknowledgements
References
Chapter 7 Summary and Outlook
Perspective
References
Subject Index
